TY - BOOK AU - Simon,Melvin I AU - Crane,Brian R AU - Crane,Alexandrine TI - Two-component signaling systems T2 - Methods in enzymology SN - 0123738512 PY - 2007/// CY - San Diego, Calif. PB - Academic Press KW - Cellular signal transduction KW - Chemotaxis KW - Signal Transduction N1 - Includes bibliographical references and indexes; Pt. A. Computational analyses of sequences and sequence alignments -- Biochemical and genetic assays of individual components of signaling systems -- Physiological assays and readouts -- Pt. B. Structural approaches -- Reconstitution of heterogeneous systems -- Intracellular methods and assays -- Genome-wide analyses of two-component systems -- Pt. C. Characterizing cross-talk In Vivo: avoiding pitfalls and overinterpretation -- Inference of direct residue contacts in two-component signaling -- Computational modeling of phosphotransfer complexes in two-component signaling -- Kinetic studies of the yeast His-Asp phosphorelay signaling pathway -- Purification of MBP-EnvZ fusion proteins using an automated system -- Measurement of response regulator autodephosphorylation rates spanning six orders of magnitude -- Transmembrane receptor chimeras to probe HAMP domain function -- Light-activated bacterial LOV-domain histidine kinases -- Characterization of bacteriophytochromes from photosynthetic bacteria: histidine kinase signalling triggered by light and redox sensing -- Biophysical assays for protein interactions in the Wsp sensory system and biofilm formation -- High-throughput screening of bacterial protein localization -- In Vitro and In Vivo analysis of the ArcB/A redox signaling pathway -- Potassium sensing histidine kinase in Bacillus subtilis -- Two-component systems and regulation of developmental progression in Myxococcus xanthus -- Two-component signalilng to the stree MAP kinase cascade in fission yeast -- Genetic and biochemical analysis of the SLN1 pathway in Saccharomyces cerevisiae -- Analysis of mitogen-activated protein kinase phosphorylation in response to stimulation of histidine kinase signaling pathways in Neurospora -- Biochemical characterization of plant hormone cytokinin-receptor histidine kinases using microorganisms using microorganisms -- Characterization of pseudo-response regulators in plants -- Reversible histidine phosphorylation in mammalian cells : a teeter-totter formed by nucleoside diphosphate kinase and protein histidine phosphatase 1 -- Histidine phosphorylation in histones and in other mammalian proteins ER -